关键词: ELISA , 检测试剂盒, 比较研究, 小鼠肝炎病毒

Abstract: Objective To compare the sensitivity, specificity, precision and reliability of 2 domestic and 1 imported ELISA kits for the detection of mouse hepatitis virus (MHV) antibody, so as to screen out a kind of economic and reliable ELISA kit. Method Three kinds of ELISA kits were chosen to detect MHV antibodies and other 5 kinds of viruses such as Sendai virus (SV), Peneumonia virus of mice (PVM), Minute virus of mice ( MVM), Poxvirus of mice ( MPV) and Reovirus type Ⅲ ( Reo-3) , and compared their sensitivity, specificity, precision and reliability. Result There were significant differences in the sensitivity of the 3 kinds of kits. Domestic kit B is 400 times as sensitivity as domestic kit A, and the imported kit is 4 times sensitivity of kit B. There were no cross reaction with SV, PVM, MVM, MPV and Reo-3 in domestic kit B and the imported one, but there were cross reactions with domestic kit A. The precision test showed that the average coefficient of variation in the imported kit was 5. 667%, and in the domestic kits A and B were 13. 825% and 13. 827%. The reliability test showed that the coincidence rates of positive and negative tests of domestic kit B and the imported one were both 100%, and the coincidence rates of positive and negative tests of domestic kit A were 40% and 80%, respectively. Conclusion The specficity and reliability of domestic kit B are consistent with the improted one, but with lower sensitivity and precison than it. Domestic kit A is lower in all tests than others.

Key words: ELISA kit, comparative study, mouse hepatitis virus