Abstract: Objective In this study, wild-type and Caveolin-1 ( Caveolin-1) knockout mice were induced by 10% carbon tetrachloride ( CCl₄) olive oil solution to establish liver fibrosis model. The effect of Caveolin-1 knockout on liver injury and collagen deposition was analyzed to explore the role of Caveolin-1 in liver fibrosis. Method Wildtype mice and Caveolin-1 knockout mice were intraperitoneally injected with 10% CCl₄ olive oil solution at 1 μL / g body weight, twice a week. Five mice were sacrificed on day 1, 3, 7, 14 and 28 after the injection. Serum and liver were collected, and the degree of liver injury was analyzed by HE staining and serum ALT and AST levels. The liver collagen deposition, together with the mRNA and protein expression of α-SMA, CollagenⅠ- and Collagen-Ⅲ were observed by Sirius red staining, Real-time PCR and Western blot to analyze the degree of liver fibrosis. Result On day 3, the contents of ALT and AST in serum of the knockout mice were significantly increased ( P<0. 01) . On day 3, 7 and 14, the necrotic area of liver of knockout mice was significantly increased ( P<0. 01 or P<0. 05) . On day 7, 14 and 28, the collagen staining area in Caveoin-1 knockout mice increased significantly ( P<0. 01 or P<0. 05) . Hepatic stellate cell activation marker, α-SMA mRNA significantly increased on day 3 and day 14 ( P<0. 05) . mRNA expression of Collagen- Ⅰ and Collagen-Ⅲ rise significantly on day 14 ( P <0. 01) . The protein expression levels of α-SMA, Collagen- Ⅰ and Collagen-Ⅲ were significantly higher in Caveolin-1 knockout mice ( P<0. 01 or P<0. 05) . Conclusion Caveolin-1 inhibited the liver inflammatory injury and fibrosis in mice induced by CCl₄.