Abstract: Objective An animal model of acute superior mesenteric artery embolism was established to evaluate the effect of self-developed thrombectomy and thrombectomy. Method Animal autologous blood clots were prepared in vitro. Interventional method were used to inject autologous blood clots into the superior mesenteric artery, resulting in arterial embolism. Embolization was performed using a thrombectomy device. Result Laboratory pigs successfully established acute intestinal ischemia-induced intestinal ischemia models, which are closer to the actual clinical situation than traditional method. Conclusion Using this experimental method, an animal model of acute superior mesenteric arterial embolism suitable for follow-up thrombectomy can be successfully established, which can better simulate the embolism of clinically-onset patients.