Abstract:

Abstract: Objective To observe whether hydrogen gas can ameliorate the allergic rhinitis ( AR ) in mice.Method Twenty-four female BALB / c mice ( 7-weeks-old) were randomly divided into the normal group, the model group, the nitrogen-oxygen group, and the hydrogen-oxygen group. Except the normal group, mice from the other 3 groups were intraperitoneally injected with 0. 3 mL saline dissolved with 50 μg OVA and 2 mg aluminum every 2 days lasting for 2 weeks. From day 15 to day 21, 10 μL saline with 200 μg OVA was administered to both nostrils. During the whole process, a mixed gas of 66. 7% N2 and 33. 3% O2 was given for 2 hours every day to the mice of the nitrogen-oxygen group, while those from the hydrogen-oxygen group inhaled a mixed gas of 66. 7% H2 and 33. 3% O2 at the same time. The number of nose scratching and sneezing of each mice was counted within 10 minutes after nasal administration from day 15 to day 21. On the 21st day, mice of each group were sacrificed, and the histological sections of the nasal cavity with hematoxylin-eosin staining ( HE) staining was performed with the nasal mucosal eosinophils counted. Result After modeling of AR, the number of nose-scratching in the model group and the nitrogen-oxygen group was increased compared with that the normal group with the difference being statistically significant ( P<0. 05) . Meanwhile, the number of nose-scratching in the hydrogen-oxygen groups was not statistically significant from that of the normal group ( P > 0. 05) . The numbers of sneezing and nasal mucosal eosinophils in the model group were significantly increased compared with those of the normal group ( P < 0. 05) . Meanwhile, the numbers of sneezing and nasal mucosal eosinophils in the hydrogen-oxygen groups were significantly lower than those in both the model group and the nitrogen-oxygen groups ( P<0. 05) . Conclusion Hydrogen gas could alleviate the allergic rhinitis in mice.