Abstract:

Abstract: Objective To explore the effect of PTEN over expression mediated by Celastrus orbiculatus extracts on the chemical sensitivity of esophageal cancer cells. Method The model mice of esophageal cancer were constructed, and they were treated intratumoral with Cel. orbiculatus extracts ( 1, 5 mg / kg ) and cisplatin (5 mg / kg) alone or in combination. The tumor volume was observed and the tumor weight was detected at 30 days. Immunohistochemistry detected the expression of Ki67, VEGF and Caspase 3. HET-1 A was treated with different doses of Cel. orbiculatus extracts (0. 5, 1, 2. 5, 5, 10, 20, 50, 80 and 100 μmol / L) for 24 h, and cell viability was determined by CCK8 assay. After pretreatment of EC109 / DDP cells with cisplatin ( 4 μg / mL ) , EC109 / DDP cells were treated with different doses of Cel. orbiculatus extracts ( 1, 2. 5 and 5 μmol / L ) . The control group was replaced with PBS instead of cisplatin. CCK8 was used to detect cell survival. The transwell was used to detect the invasion of cells. The expression of EMT-related protein and apoptosis-related protein was detected by Western blot. The apoptosis of cells was detected by Hoechst staining. Result After treatment with Cel. orbiculatus extracts ( 1 mg / kg and 5 mg / kg ) , the tumor weight and volume in the model mice were significantly reduced ( P< 0. 05) . The expression level of PTEN protein was significantly increased ( P < 0. 05) . Ki67, VEGF and Caspase 3 positive cells were significantly reduced ( P<0. 05) . Compared with the control group, the survival rate and invasiveness of EC109 cells were significantly decreased ( P < 0. 05 ) . Ki67, PCNA, ncadherin and Vimentin were significantly decreased ( P < 0. 05 ) . The expression levels of e-cadherin, cleaved PARP and cleaved caspase 3 protein and the apoptosis rate were significantly increased ( P <0. 05) . Cell survival rate and invasion force were significantly increased after PTEN interference ( P < 0. 05) . Apoptosis rate decreased significantly ( P < 0. 05) . Conclusion PTEN overexpression mediated by Cel. orbiculatus extracts enhanced the inhibitory effect of cisplatin on EC109 growth, invasion and EMT of esophageal cancer cells, promoted cisplatin induced EC109 apoptosis of esophageal cancer cells, and thus enhanced the chemical sensitivity of cisplatin to esophageal cancer cells.