Abstract:

Abstract: Objective Glycosylated hemoglobin ( HbA1c) is an indicator commonly applied to clinical medicine to monitoring blood glucose control status. To investigate whether HbA1c can be used to monitoring the blood glucose control status of miniature pigs, we detected the blood HbA1c ratio in diabetes mellitus research in Bamaminipigs, so as to provide fundamental data for the minipigs using in diabetes mellitus researches. Method Fourteen Bama minipigs were divided randomly into 3 groups, which were control, HFD+STZ and HCFD+STZ group respectively. The animals in control group were fed with standard diet, and that in HFD+STZ and HCFD+STZ groups were fed with HFD diet or HCFD diet separately. After 3 months, STZ of 90 mg / kg were administered by intravenous injection. Then the pigs continued to be fed with HFD or HCFD diet. During the test, serum GLU, INS, HbA1c, GST, GLT, BUN and CRE were detected monthly. Result Four fifth of minipigs in HFD+STZ group and 4 / 5 of minipigs in HCFD + STZ group got significantly high serum glucose concentrations after injection of STZ, and sustaining to the end of the research. Nevertheless, their HbA1c ratios were not changed significantly during the
research and no apparently differences among the groups. The reason that the HbA1c ratios did not raised with the increase of serum glucose is presumed the badly permeability of pig erythrocyte for glucose. Conclusion To detect HbA1c ratio is not useful to monitoring serum glucose levels in minipigs.