Abstract:

Abstract: Objective To establish a mouse model that simulates myocardial infarction ( MI) in postmenopausal women. Method Female mice were ovariectomized ( OVX) and occluded the left anterior descending coronary artery to induce myocardial infarction. We evaluated the changes in mouse heart function and myocardial infarction area, and the degree of myocardial fibrosis by ultrasound assessment, hemodynamic analysis, morphological observation, immunohistochemistry and TUNEL staining. Result OVX combined with MI treatment can aggravate cardiac function impairment, significantly increase myocardial fibrosis and myocardial infarction area, induce cell apoptosis and TGF-β1 expression, and significantly increase serum pro-inflammatory cytokines levels, such as TNF-α and IL-10. These mice displayed thinning of the ventricular walls, dilation of the ventricles and remodeling of the heart. All these symptoms consistent with the menopausal women after MI. Conclusion The mouse model that mimics the occurrence of MI in postmenopausal women were successfully established, its function and histological characteristics were evaluated. It provided an ideal animal model for the study of heart protection in menopausal women.