关键词: 裸鼠, 皮下移植瘤, 小动物超声, 超声微泡, 肿瘤血管

Abstract: Objective To observe the angiogenesis of human bladder cancer ( BT-B ) and human liver cancer ( Huh7) subcutaneously transplanted tumors by high frequency ultrasound in small animals. To evaluate the value of contrast-enhanced ultrasound in tumor angiogenesis. Method Two subcutaneous transplantation tumor models of human BT-B bladder cancer and human Huh7 liver cancer were established by subcutaneous injection. The number and size of small blood vessels were observed by confocal laser probe in vivo. The expression of CD31, a tumor vessel marker, was observed by immunohistochemistry and the microvascular density was calculated. Result Both transplanted tumors showed good growth patterns. B-mode ultrasound showed that both Huh7 and BT-B subcutaneous tumors were heterogeneous hypoechoic nodules. Color doppler mode and spectral doppler mode showed that the blood flow signal and velocity of BT-B subcutaneous tumor were higher than that of Huh7 subcutaneous tumor. Contrast-enhanced ultrasound showed that the subcutaneous tumor of Huh7 presented diffuse and uneven enhancement, and the peripheral ring or semi-ring enhancement of the subcutaneous tumor of BT-B presented enhancement toward the center of the lesion, which was consistent with the probe in vivo laser confocal result and pathological result . Peak intensity of CEUS parameters ( PI) was positively correlated with MVD ( r = 0. 844, P < 0. 05) . Conclusion CEUS can better display the distribution of tumor blood vessels, and PI is positively correlated with pathological MVD, suggesting the proliferation of tumor blood vessels, which can be used as a reliable method for noninvasive evaluation of tumor angiogenesis.

Key words: nude mice, subcutaneously transplanted tumors, ultrasound in small animals, ultrasound microbubbles, tumor vessels