关键词: 雪貂, 阿留申病病毒, PCR, 特异性, 敏感性

Abstract: OBJECTIVE To establish a PCR detection method for Aleutian virus for the detection of ferrets Aleutian virus in experimental animals. Methods A pair of primers were designed with reference to the VP2 gene sequence of Aleutian disease virus in Genebank. The PCR method was established to test the specificity and sensitivity, and the experimental samples of Ferrets feces were screened. RESULTS The sequencing results showed that the designed primers can specifically amplify the 531 bp gene fragment of VP2 of Aleutian disease virus; There were no unspecific amplification when the following experimental animal DNA virus were used as templates in ADV PCR reaction: mouse Polyoma Virus, Minute virus of mice, Canine Parvovirus, Herpes Simplex virus, Ectromelia virus, Sendai virus, Mouse adenovirus; the nucleic acid plasmid with a sensitivity of 90.6 copy/μL was obtained. A total of 39 samples of ferrets were tested and no Aleutian virus nucleicacid was detected. Conclusion The current ADV PCR detection method shows applicable specificity and sensitivity, which can be used as a pathogen detection method in the screening of ferrets Aleutian disease virus in experimental animals.

Key words: Ferret, Aleutian disease virus, PCR, specificity, sensitivity