实验动物科学 ›› 2019, Vol. 36 ›› Issue (06): 27-.

• 研究报告 • 上一篇    下一篇

Hartley豚鼠SPF级核心种群生长曲线与血液生理生化指标测定

  

  1. 中国食品药品检定研究院
  • 出版日期:2019-10-28 发布日期:2020-09-09

Determination of Growth curve and Hematological Parameters of SPF Expansion Stock Hartley Guinea pigs

  • Online:2019-10-28 Published:2020-09-09

摘要: 目的 测定SPF级Hartley豚鼠核心群的生长体质量,血液生理、生化指标,并与清洁级豚鼠各指标进行比较。方法 选择种群中雌、雄个体各10只,通过称重,记录0~8周龄体质量;豚鼠采血,应用全自动血细胞计数仪与全自动血液生化分析仪检测各项生理、生化指标。结果 在SPF级豚鼠血液生理指标中,白细胞计数(WBC)、中性粒细胞计数(NEUT)、嗜酸性粒细胞计数(EOS) 3项指标,在雌性与雄性间有显著性差异(P0. 05)外,其他指标均存在极显著差异(P<0. 01)。结论 绘制了SPF级豚鼠生长曲线。SPF级豚鼠血液学指标在性别间有差异,与清洁级豚鼠相比存在显著差异。

关键词: 豚鼠, SPF级, 生长曲线, 血液生理, 血液生化

Abstract: Measured the growth weight and blood physiological & blood biochemical parameters in SPF Hartley guinea pig core population. Compare with the hematological parameters of clean guinea pig. The results show that the WBC、NEUT、EOS have significant difference (P<0.05), the PCT、MPV、LYM、LYM%、NEUT%、EOS%、BAS% have very significant difference(P<0.01)between SPF male and female in blood physiological parameters. The ALP has significant difference(P<0.05),the 3 indexes as BUN、CREA、CHO have very significant difference(P<0.01)between SPF male and female in blood biochemical parameters. The results of comparison with CL guinea pigs show that WBC between SPF and CL of both sex, MCV、PLT between SPF and CL of female have no significant difference(P>0.05),other indexes have very significant difference (P<0.01)between SPF and CL guinea pigs. Conclusion, the growth curve of SPF guinea pig was plotted. The hematological parameters of SPF guinea pigs are significantly different from sex and have very significant difference between SPF and clean guinea pigs.

Key words: guinea pig, SPF, growth curve, blood physiological parameters, blood biochemical parameters