关键词: 慢性肝损伤, BALB/c小鼠, 周期性病变

Abstract: Objective To observe changes in liver damage induced by carbon tetrachloride (CCl4)induced chronic liver disease in mice.Method One week after normal feeding in normal BALB/c mice,0.5% CCl4 solution (10 μL/g,one time every three days for 10 weeks,lasting 10 weeks)was intraperitoneally injected.After 2,4,6,8,and 10 weeks of injection,the serum ALT and AST were detected,HE and Masson staining after liver fixation,observation of liver structure,cell morphology and fibrosis degree in mice.Result When CCl4 solution was injected for 2 weeks,the liver cell damage was mainly denatured with a large number of inflammatory cells infiltration and a small amount of collagen deposition,and ALT and AST were slightly elevated;At 4 weeks,hepatocyte injury was mainly necrosis (P<0.01),and the number of hepatocytes (P<0.01)and inflammatory cell infiltration decreased (P<0.01).Collagen deposition developed into stripe collagen fiber deposition,and ALT and AST increased significantly(P<0.01);At 6 weeks,necrotic hepatocytes were significantly reduced (P<0.01),the number of hepatocytes increased (P<0.01),the infiltration of inflammatory cells was further reduced (P<0.01),collagen fiber deposition was transformed to collagen deposition (P<0.05),ALT and AST were basically normal(P<0.01));At 8 weeks,the degeneration of hepatocyte was increased again,with a large number of inflammatory cell infiltration (P<0.01),and a slight increase in ALT and AST;At 10 weeks,hepatocyte injury was mainly necrosis (P<0.01).The number of hepatocytes (P<0.01)and inflammatory cell infiltration decreased (P<0.05).Collagen deposition developed into stripe collagen fiber deposition,and ALT and AST increased significantly (P<0.01).Conclusion The chronic liver injury model of mice induced by 0.5%CCl4 solution has obvious and regular liver injury cycle changes,and the degeneration stage,necrotic stage and remission stage of hepatocyte appear in turn.

Key words: Chronic liver injury, BALB/c mice, periodic lesions