关键词: 牛疱疹病毒Ⅰ型, 实时荧光定量PCR, 牛源性样本

Abstract: Objective To establish a real-time fluorescent quantitative PCR method for detection of Bovine herpesvirus type 1 (BHV-1)in Bovine origin samples.Method The primers and TaqMan probe were designed and synthesized according to the published BHV-1 specific sequences of gB gene.Q-PCR method is established.Then carries on the specificity,sensitivity,repeatability and stability of this method were tested.The method is used to detect 181 Bovine origin samples.Result The developed Q-PCR method was no cross reaction with Bovine parainfluenza virus type 3 (BPIV3),Bovine viral diarrhea virus type 1(BVDV1),herpes virus type 1 (HSV-1),Felid herpesvirus 1 (FHV-1),and Pig pseudo rabies virus (PRV);sensitivity was 1×101 copies/μL;Coefficient of variation(CV)was less than 5%;There were 6 positive reaction detected in the 181 Bovine origin samples.Conclusion The developed PCR method is good in specificity,sensitivity,repeatability and stability and may be used for rapid quantitative detection the BHV-1 in Bovine origin samples.

Key words: Bovine herpesvirus type 1, Real-time fluorescent quantitative PCR, bovine Origin Sample