关键词: 小鼠, 静脉血细胞悬液, 放置时间, 血常规检测

Abstract: Objective To investigate the effect of storage time on blood routine test of venous haematocyte suspension in mice．Method Twenty adult ICR mice and 20 C57BL/6 J mice were randomly selected,which were adopted venous blood.Collection each of quantitative venous blood added in diluent which were adequate for quantitative blood cell analytical instrument to be mixed to make venous haematocyte suspensions．Then detected by trace blood determination on 0 min,15 min,30 min,1 h,2 h,4 h,6 h,8 h in the MEK-6410C full-automatic blood cell analyzer．Result The change trend of blood routine data in ICR mice and C57BL/6 J mice is basically the same．Compared with 0 min,RBC decreased significantly at 2 h,4 h,6 h and 8 h(P0.05).Conclusion By trace blood determination,the blood routine test of mice is done as soon as possible after the haematocyte suspension is made．The RBC is better to be measured in 1 h,HCT,MCV related to RBC were tested should not be more than 30 min.The PLT is better to be measured in 2 h,PCT,MPV related to PLT were tested should not be more than 1 h,The data of WBC and the content of HGB are stable and should be completed within 8 h.

Key words: mouse, venous haematocyte suspension, storage time, blood routine test