关键词: 小鼠, 脑脊液, 方法

Abstract: Objective To establish a simple method for drawing out the CSF of mice and provide references for relative researches. Method First place the mouse in the position that the head forms 120°, shave the skin and separate the subcutaneous tissue and muscle bluntly. As a result , the dura mater of the cisterna magna appears clearly. Penetrate the white dura mater and then reach the cerebellomedullary cistern by the capillary tube. Obtain the CSF and withdraw the tube. Finally, suture the mouse. Result 2—5 μL CSF samples can be collected successfully with mice survived. Conclusion Compared with others, the method we established is proved to be with cheaper material, higher success rate and survival rate.

Key words: mouse, Cerebrospinal fluid, method