关键词: 动脉粥样硬化, ApoE-/-小鼠, 血脂

Abstract: Objective To study the effect of western diet and standard diet on the blood fat and aortic arch pathology of apolipoprotein E gene knock out mice. Method We feed 3 months old ApoE-/- mice with western dietary or standard diet for 3 months, 4 months and 5 months, respectively, then collect peripheral blood and aortic arch. We use Automatic blood analyzer to detect lipid level in serum, and observe the formation of atherosclerotic plaque through sectioning and staining.Result On the blood lipids detection, total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) in serum of mice feeding western diet and standard diet were significantly higher than the control group. In addition to HDL-C mice 4 months feeding, the lipid index of western diet lipid groups are significantly higher than the standard diet group on the same age. On the stained sections, western diet aortic plaque and incidence of atherosclerosis are significantly increased.Conclusion Both Western dietary and standard diet can induce ApoE-/- mice showed AS symptoms, the effect of western dietary is more obvious.

Key words: Atherosclerosis, ApoE-/- mice, blood lipid