关键词: 脱氢表雄酮, 卵母细胞质量, 超排卵

Abstract: Objective To investigate the effect and mechanism of DHEA on the quality of oocytes of C57BL/6J mice after superovulation. Method Thirty C57BL/6J mice at the age of 3 weeks were randomly divided into 3 groups, which were control group、low does group(2 mg/kg·d-1)and high does group(5 mg/kg·d-1), respectively. DHEA was administrated via the gastrointestinal tract for one week. Oocytes were collected after superovulation and underwent IVF. qRT- PCR was used to detect gene expression of Grem1、Has2、Ptgs1、Ptgs2 and Vcan，the makers of cumulus cells collected after superovulation. Gene expression of Caspase3 and Bcl2l10 were also detected by qRT-PCR. Result After DHEA treated, IVF showed that the proportion of heterotypic eggs decreased in the experimental group. The transcription levels of Has2, Ptsg1 and Vcan in cumulus cells of experimental group were up-regulated compared with those of control group, while the mRNA levels of Germ1 and Ptgs2 were down-regulated. The mRNA level of Caspase3 was down regulated and the Bcl2l10 mRNA level was unregulated. Conclusion After DHEA treatment, the apoptosis of cumulus cells can be inhibited, and the quality of oocytes can be improved.

Key words: DHEA, Quality of oocytes, Superovulation