实验动物科学 ›› 2019, Vol. 36 ›› Issue (04): 1-.

• 研究报告 •    下一篇

高糖高脂饲料诱导的小型猪肥胖模型

  

  1. (1. 中国农业大学动物医学院,北京 100093)(2. 中国人民解放军总医院医学实验动物中心,北京 100853)
  • 出版日期:2019-08-28 发布日期:2020-09-10

High Fat and High Sugar Diet Induced the Fat Model of Bama Miniature Pigs

  • Online:2019-08-28 Published:2020-09-10

摘要: 目的 探讨高脂高糖饲料对巴马小型猪脂肪分布与血液生化指标的影响。方法 巴马小型猪14头,实验组8头,为本实验室培育的2型糖尿病易感小型猪,饲喂高脂高糖饲料30周;对照组6头饲喂正常饲料。30周后测定体型系数、CT扫描脂肪含量、采集血液检测生化指标,比较实验组与对照组的差异。结果 高脂高糖饲料饲喂30周后实验组动物与对照组动物在体质量、体质指数(BMI)、脂肪总含量差异显著(P<0.05),内脏脂肪含量差异极显著(P<0.01);血液生化指标中胰岛素、总胆固醇、高密度脂蛋白胆固醇、甘油三脂与对照组相比差异极为显著(P<0.01),低密脂蛋白胆固醇与对照组相比差异显著(P<0.05)。此外,实验组巴马小型猪血清蛋白、肌酸酐与对照组差异显著(P<0.05);尿酸、尿素与对照组差异极显著(P<0.01),显示实验组小型猪呈现肾损伤。结论 高脂高糖饮食能够引起巴马小型猪脂肪堆积,内脏脂肪堆积尤为明显,血液生化指标呈高脂血症伴有肾功能的损伤。

关键词: 巴马小型猪, 脂肪分布, 血液生化, 2型糖尿病

Abstract: Objective The effects of high fat and high sugar diet(HFHSD) on fat distribution and blood biochemical parameters in Bama miniature pigs. Method Fourteen Bama minipigs were divided into two groups. Experimental group pigs fed with high fat and high sugar diet for 30 weeks.Six Bama miniature pigs in control group were fed with normal feed. After 30 weeks, body mass index, fat distribution and biochemical indexes of blood were measured. Result The body weight, body mass index (BMI) and total fat content especially visceral fat content increased significantly in experimental group.Blood biochemical indicators of insulin, total cholesterol, high and low density lipoprotein cholesterol, blood creatinine, uric acid and urea were increased significantly compared with control group. Conclusion High fat and high sugar diet can cause fat accumulation in Bama miniature pigs, especially visceral fat.Blood biochemistry is characterized by hyperlipidemia and impaired renal function.

Key words: Bama miniature pig, fat distribution, blood biochemistry, type 2 diabetes mellitus