关键词: 脉络膜新生血管, 532 nm激光, 激光功率, 棕色挪威大鼠

Abstract: Objective To induce the choroidal neovascularization model of Brown Norwegian rats with different power of 532 nm laser,we would explore the highest fluorescein leakage rate by the best laser power. Method Forty-five Norwegian rats were randomly divided into three experimental groups: 15 rats in each group. The laser power were 400 mW, 300 mW, 200 mW, the spot diameter was 100 μm, the exposure time was 100 ms, and each eye was lasered 10～12 points. The experimental datas were collected from the 1st～4th week after lasering Brown Norway. The experimental datas were statistically analyzed by fundus fluorescein angiography and optical coherence tomography. Result The retina of the fundus showed that the retina had edema due to the destruction of the vitreous membrane after fluorescence induction. FFA showed that the the leakage rate of the laser power of 200 mW compared with 300 mW and 400 mW at the 1st to 4th was statistically significant (P0.05), and the leakage rate at the 2nd and 4th weeks was statistically significant (P0.05).But there was significant difference between the 1st and 3rd week of 200 mW laser power(P0.05). Optical coherence tomography showed retinal was disordered, then retinal begained to form choroidal neovascularization.The choroidal neovascularization was ultimately stabilized,and part of the retinal structure atrophy thinning. Conclusion 200 mW, 300 mW and 400 mW laser power of 532 nm laser can induce the formation of choroidal neovascularization model by Brown Norway. The formation rate of 300 mW laser power is the highest.The choroidal neovascularization begins to occur after the 1st week, reaching the peak in the 3rd week, and the choroidal neovascularization tends to be stable at the 4rth week.

Key words: choroidal neovascularization, 532 nm laser, laser power, Brown Norway