关键词: 长爪沙鼠, 2型糖尿病, ND3, NF-κB, 免疫组织化学

Abstract: To investigate the localization and expression of NADH dehydrogenase 3 (ND3) and nuclear factor κB (NF-κB) in livers and kidneys of spontaneous hereditary diabetic and non-diabetic gerbils. We selected 6 diabetic and 6 non-diabetic control gerbils, and collected livers and kidneys from each animal, respectively. Immunohistochemistry was conducted to localize and semi-quantify ND3 and NF-κB in diabetic and non-diabetic control groups. Both ND3 and NF-κB were expressed in hepatocytes. And compared with the control group, NF-κB expression in livers was elevated significantly in the diabetic group. ND3 was also localized in renal tubular epithelial cells of kidneys. And ND3 was up-regulated in kidneys of diabetic gerbils, relative to the control gerbils. In conclusion , ND3 expressed in renal tubular epithelial cells was significantly up-regulated in kidneys of diabetic gerbils, and NF-κB expressed in hepatocytes was also increased in livers of diabetic gerbils, suggesting that the aberrant expression of ND3 in kidneys and NF-κB in livers may relate with the initiation and development of diabetes in gerbils.

Key words: Mongolian gerbils Type 2 diabetes, ND3, NF-κB, Immunohistochemistry