关键词: 失眠, 安神脐贴, 自主活动, γ-氨基丁酸, 去甲肾上腺素

Abstract: Objective To explore the effect of Anshen umbilical paste on autonomic activity and serum levels of gamma aminobutyric acid (GABA) and noradrenaline (NE) in experimental insomnia rats, in order to reveal its mechanism. Method The model of insomnia rats were established by 4-Chloro-DL-phenylalanine (PCPA) injected intraperitoneally. Forty rats were randomly divided into blank control group, model control group, Anshen umbilicus plaster (2.70 g/kg) group and diazepam (0.90×10-3g/kg) group. The general condition and autonomic activity of the rats were observed, and the contents of serum GABA and NE were detected by enzyme linked immunosorbent assay (ELISA). Result Compared with the model control group, the body weight of the rats in Anshen umbilicus plaster group and diazepam group were significantly increased (P<0.05), and the distance and speed of the movement were significantly decreased (P<0.05 or P<0.01), and the frequency and residence time in the central area also declined. After the treatment with Anshen umbilical paste and diazepam, the content of GABA in serum of rats were increased significantly (P<0.01), and the content of NE were decreased significantly (P<0.01). Conclusion Anshen umbilical paste could improve the general condition of insomnia rats and reduce the autonomic activity. The mechanism of promoting sleep may be related to the increase of GABA secretion and the decrease of NE release.

Key words: insomnia, Anshen umbilical paste, autonomic activity, GABA, NE