关键词: 鸭, 主要组织相容性复合体, 无特定病原体, 单倍体型, 抗原处理相关转运体

Abstract: Objective Optimization of genetic testing technique for MHC haploid duck. Method Based on the specific pathogen free (SPF) ducks nurtured and preserved in the National Poultry Laboratory Animal Seed Center and polymorphism of duck double-copy gene (Tap1 and Tap2) of the transporter associated with antigen processing (TAP) located in the core region of major histocompatibility complex (MHC). MHC haplotype SPF ducks were selected by using Tap1 genome short tandem repeat (STR), Tap2 peptide binding region sequence and Tap2 genomic sequence genotyping, respectively. Result The STR result of F0 generation and F1 generation ducks were completely consistent. The sequences of Tap1 and Tap2 of F2 and F3 generation duck were completely homozygous. Six consecutive generation Tap2 genome sequence analysis shows that B3 group of ducks have two mutations, B1, B2 and B4 group of ducks have genetic stability. Conclusion Four MHC haploid SPF ducks B1, B2, B3 and B4 were successfully established, which provided laboratory animal support conditions for further research on immune genetics of ducks.

Key words: duck, MHC, SPF, haploid, TAP