关键词: 杏鲍菇, 菌糠, 多糖, 超声提取, 免疫作用

Abstract: Objective To observe the role of polysaccharide from pleurotus eryngii bacterial chaffs on spleen index，spleen lymphocyte proliferation and immune factor of mice. Method The ultrasonic extraction of polysaccharide from pleurotus eryngii bacterial chaffs were mixed with the basic feed, which was made into low dose (200 mg/kg), medium dose (400 mg/kg) and high dose (800 mg/kg) feed. Forty eight KM mice were randomly divided into four group, including blank group, low dose group, medium dose group and high dose group. After 60 days of continuous feeding, the index of spleen, the proliferation of splenic lymphocytes and the content of IFN-γ, TNF-α and IL-6 were measured to study the immune regulation effect of polysaccharide on mice. Result Compared three kinds of polysaccharide groups with blank control group,the low dose group could significantly increase the spleen index (P<0.05),the medium dose group could significantly increase the spleen index and spleen lymphocyte proliferation in mice (P<0.05).In addition, it could increase the content of TNF-α and IL-6 (P<0.05); High-dose group could significantly increase the content of IFN-γ (P<0.05) and which also could significantly increased the spleen index,the spleen lymphocyte proliferation, the content of TNF-α and IL-6 in mice (P<0.01). Conclusion The polysaccharide from pleurotus eryngii bacterial chaffs play important role on regulating spleen index，spleen lymphocyte proliferation and Immune factor in mice.

Key words: pleurotus eryngii, bacterial chaff, polysaccharides； ultrasound extraction, immunization