关键词: 铜绿假单胞菌, 甲醛, 气溶胶, 动态暴露, 动物模型

Abstract: Objective Establish the respiratory system injury model of rat by inhalation formaldehyde combining with microbial aerosol. Method Thirty one wistar rat (180±10 g,8 w) were divided into four groups, separately growth under normal condition (group CO28), exposure under(2.5±0.2)ppm formaldehyde (group FA28), exposure under(1×107)cfu/m3 PAO1 (group PAO), and exposure under (3.4±0.3) mg/m3 formaldehyde combining with PAO1 aerosol (group FA&PAO). The animal morphology injury of lung tissue was observed by HE staining, the apoptosis of lung epithelial cells was observed by Tunnel chromogenic in situ detection. The peripheral blood were collected to examination IgM、IL-1β、IL-8、TNF-α、CC10 concentration and SOD activity. Result Morphological observation showed that the lung tissue of three experimental groups was changed with alveolar septum widened, interstitial edema and inflammatory cell infiltration and the most obvious changes were detected in co-exposure groups. Tunnel tests also showed that more apoptotic cells were detected in the lung epithelium cell in co-exposure groups. In contrast to those of normal control groups, after inhalation of microbial aerosol, rat’s serum IgM、 TNF-α、IL-1β、IL-8 levels increased significantly, with more significantly increasing in co-exposure groups(P0.05). Conclusion Formaldehyde combining with microbial aerosol co-exposure induced synergistic damage of rat lung tissues and destruction the scavenging ability of lung tissue to heterogeneity pollutants.

Key words: Pseudomonas aeruginosa, formaldehyde, aerosol, dynamic exposure, animal model