关键词: 肝癌, PDOX模型, 近红外荧光, 分子影像

Abstract: Object A patient-derived orthotopic xenograft PDOX model was established to study the application of near-infrared fluorescence (NIRF) in vivo imaging and PET/CT in model evaluation. Method The PDX model of hepatocellular carcinoma was established by subcutaneously injecting clinical fresh hepatocellular carcinoma (HCC) specimens into severely immunodeficient NPG mice. The PDX model was evaluated by histomorphometric observation, STR genotyping, and immunohistochemistry. PDX models were implanted in nude mice to establish PDOX models by orthotopic liver transplantation. Near-infrared fluorescent dye IR-783 was injected to detect the occurrence of tumors by in vivo imaging of small animals. 18F-FDG was injected through the tail vein and observed by small animals PET/CT to confirm liver orthotopic tumor growth. Result The STR genotyping result showed that the human characteristics, histomorphological observation and immunohistochemical detection of PDX tumors indicated that PDX tumors retained the pathological features of primary tumors; detection of liver tumors was detected by near-infrared fluorescence in vivo imaging; PET/CT was available. The enrichment of 18F-FDG molecular probes in the mouse liver was clearly observed. Conclusion The PDOX model of hepatocellular carcinoma was established successfully. The model can be evaluated by living animal imaging and PET/CT imaging technology. It provides a good animal model for the treatment and pathogenesis of hepatocellular carcinoma.

Key words: Liver cancer, PDOX model, Near-infrared fluorescence, molecular imaging