关键词: 大鼠, 亚急性, 酒精性, 肝损伤模型

Abstract: Objective To determine the most convenient conditions for the establishment of subacute alcoholic liver injury by comparing different doses of alcohol. Method The SPF female Wistar rats (150～180 g) were selected as the model group and control group. In the model group, the alcohol content was 15% and 30% respectively. The control group was given distilled water, with 15 mL/kg·BW, and the trial period was 8 weeks. At the end of the experiment, the serum glutamate aminotransferase (ALT), aspartate aminotransferase (AST) and ethanol dehydrogenase (ADH) were measured from orbital venous plexus of rats. Hepatic histopathological lesions were determined by liver tissue, and MDA, SOD, GSH, TG were measured. Result There was significant difference in MDA between 30% alcohol group and blank control group (P 0.05).Model group rats blood lipid metabolic disorder and the accumulation of fat in the liver could be founded and histologic examination showed diffuse hepatocellular fatty degeneration, formation of different size of the fat granule in the cell. According to the score of the liver disease scale, 15% alcohol, 30% alcohol group compared with blank control group, the degree of liver steatosis has significant difference (P< 0.01, P< 0.01). Conclusion Rats filled the stomach 8 weeks, using the ordinary liquor of alcohol content 30% can cause liver damage. The result can be repeated and subacute alcoholic liver injury animal model of rats is successfully established.

Key words: rats, subacute, alcoholic, liver injury animal model