益心通络胶囊体外抗凝血的生物活性测定法研究

实验动物科学 ›› 2017, Vol. 34 ›› Issue (03): 20-23.

益心通络胶囊体外抗凝血的生物活性测定法研究

( 1．北京中医药大学中药药理系，北京 100102) ( 2．北京市药品检验所，北京 100035)

出版日期:2017-06-30 发布日期:2017-09-07

Bioassay Method of Anticoagulant Ｒesearch of Yixintongluo Capsule in Vitro

Online:2017-06-30 Published:2017-09-07

摘要/Abstract

摘要： 摘要: 目的考察以凝血酶时间( TT) 为反映体系建立益心通络胶囊体外抗凝血的生物活性测定法的可行性。方法将不同浓度的益心通络胶囊浸提液加入兔血浆中，测定凝血酶时间( TT) ，观察益心通络胶囊浸提液浓度与 TT 值的量效关系。规定浓度为 0. 261 g /mL 的益心通络胶囊溶液使空白血浆( 加 0. 9% 生理盐水) 凝血酶时间延长 1 个百分点定义为 1 个效价单位( U) ，定义并测得益心通络胶囊标准品效价。结果当益心通络胶囊浸提液在 0. 1886 ～ 0. 2610 g /mL 浓度范围内时，凝血酶时间与浓度线性较好( r = 0. 9980; 0. 9985; 0. 9829) ; 测得益心通络胶囊标准品的效价为 2395 U/g。结论益心通络胶囊体外凝血酶时间的生物活性测定方法具有可行性。

关键词: <p>生物活性测定法, 凝血酶时间, 益心通络胶囊</p>

Abstract: Abstract: Objective To inspection the feasibility of bioassay method of anticoagulant research of YiXinTongLuo capsule in vitro． Method Different concentrations of aqueous extract of YiXINTongLuo capsule were mixed with Ｒabbit plasma and the TT was determined by coagulometer． One titer unit( U) was defined as one second longer for anticoagulant research of YiXinTongLuo capsule than the coagulation time of the blank control，and the titer of YiXinTongLuo capsule standard substance was defined and measured．Ｒesult Within the range of 0. 1886 ～ 0. 2610 g /mL，TT and the aqueous extract concentration showed a good linear relationship respectively( r = 0. 9980; 0. 9985; 0. 9829) ． The titer of the standard substance of YiXinTongLuo capsule was measured to be 2395 U /g． Conclusion It is feasibility that TT used toestablish bioactivity assay in vitro．

Key words: <p>bioactivity assay, thrombin time, YiXINTongLuo capsule</p>

中图分类号:

Ｒ965. 2

肖珠, 王志斌, 程源, 王立娜, 郭玉东, 胡宇驰, 曹春然. 益心通络胶囊体外抗凝血的生物活性测定法研究[J]. 实验动物科学, 2017, 34(03): 20-23.