关键词: <, p>, 体外皮肤模型, 皮肤刺激性, 替代<, /p>

Abstract: Abstract: Objective To assess the sensitivity，specificity and accuracy of an in vitro EpiDerm Skin Irritation Test． Method Thirty μL of the test substance was added into three single EpiDerm skin tissues，and negative control ( NC) and positive control ( PC) were introduced． Each group contained three parallel tissues． After the 60 min test substance exposure，MTT viability test and isopropanol extraction were done． Ｒead A value in a 96 － well plate spectrophotometer using a wavelength at 570nm，relative viability was calculated． skin irritation potential of the test material was predicted if the relative viability was below 50% ． The results of acute skin irritation test were compared with that in vivo． Ｒesult Comparing with acute skin irritation test in vivo，the sensitivity，specificity and accuracy of in vitro EpiDerm Skin Irritation Test were 100% ． And the results were objective，the workload was small． The issue of animal ethics was completely avoided． Conclusion In vitro EpiDerm Skin Irritation Test is the most ideal method of in vitro skin irritation．

Key words: <p>skin tissues, skin irritation, replacement</p>