关键词: <, p>, 稀有鮈鲫, 组织学, 切片, HE 染色<, /p>

Abstract: Abstract: Objective Gobiocypris rarus，a freshwater fish with high prolificacy，distributes in Sichuan Province． In order to became a laboratory animal，the fish has been investigated its geographical distribution，morphology， habita，ontogenesis，reproduction，growth，taxonomy，semitiviqto ecological factors，karyotype and so on by Chinese Academy of Science since 1990s． However，there was few histological background of Gobiocypris rarus until now． Here we introduce a practical way to make sections of Gobiocypris rarus． Method The healthy individuals were fixed in the Bouin’s fluid，washed with running water slightly，and put into decalcifying fluid． After finishing decalcification，the fish was cut into several small pieces，the specimens were dehydrated and infiltrated，respectively，and then waxed with paraffin． The specimens in solid paraffin were allowed to cut into serial slices． The slices were dyed by Hematoxylin and Eosin ( H＆E) staining according to the routine protocol． Transverse and sagittal section pictures were collected by a optical microscope． Ｒesult The sections were scaned under microscope and the pictures of different kinds of organs and tissues were taken． Those high quality pictures can set up the histological background of Gobiocypris rarus． Conclusion Histology can detect features of disease which are difficult to recognize from clinical symptoms． The histological background of Gobiocypris rarus tissues and organs is the foundation of pathology knowledge． The method of making sections of Gobiocypris rarus may contribute to the research of aquatic animal．

Key words: <p>Gobiocypris rarus, histology, section, HE stainin</p>