关键词: <, p>, 树鼩, 小肠上皮细胞, 酶消化, 免疫荧光, 鉴定<, /p>

Abstract: Abstract: Objective To isolate intestinal epithelial cells( IECs) from the tree shrew，to establish a cellular model for drug mechanism research，and to lay the foundation for establishment of intestinal epithelial cell lines． Method Collagenase type XI，and neutral protease type I and DTT were used for digestion of tree shrew small intestine． Cells morphology was observed by phase contrast microscopy，identified by cytokeratin Immunofluorescence assay．Ｒesult Isolated intestinal epithelial crypts attachment were performed in 24 h，confluent in 6 d． Epithelial outgrowth from isolated crypts were formed with confluent monolayers cobblestone and multi-angular morphology． Cells were passaged every 2 ～ 3 d from the nest generation，and between the third and sixth day from the day when cells were plated the cells got into the logarithmic growth phase． The cells were stained positive for cytokeratin immunofluorescence staining． Conclusion Intestinal epithelial cells were successfully isolated from the tree shrew， and the method of isolation for tree shrew’s intestinal epithelial cells was established．

Key words: <p>tree shrew, intestinal epithelial cell, enzymatic digestion, Immunofluorescence, identification</p>