关键词: <, p>, SPINK1, Spink3, EＲK, mTOＲ, 肝癌<, /p>

Abstract: Abstract: Objective To investigate the molecular mechanisms of the regulation to Spink3 expression in mouse hepatic tumors． Method The mＲNA levels of SPINK1 in human hepatocellular carcinoma and Spink3 in hepatic tumors of H-ras12V transgenic mice were detected by ＲT-qPCＲ． The protein levels of p-EＲK and p-mTOＲ and mＲNA levels of Spink3 in primary cultured hepatic tumor tissues and tumor-adjacent normal tissues of H-ras12V transgenic mice were detected by Western blot and ＲT-qPCＲ． In vivo inhibition of the activities of p-EＲK and p-mTOＲ was performed to investigate their influence on the mＲNA levels of Spink3． Ｒesult Compared with tumor-adjacent normal tissues，the mＲNA levels of SPINK1 and Spink3 were significantly higher expressed in hepatic tumor tissues ( P ＜ 0. 05) ． In the primary cultured tumor and normal liver tissues of transgenic mice，along with the cultured time ( 6，12. 24. 48 h) ，the protein levels of p-EＲK and p-mTOＲ are significantly decreased compared to the 0 h． Consistently，the mＲNA levels of Spink3 were also significantly decreased ( P ＜ 0. 01) ． Inhibition of the activities of p-EＲK significantly reduced the mＲNA levels of Spink3 ( P ＜ 0. 05) ． However， Inhibition of the activities of p-mTOＲ had no influence on the mＲNA levels of Spink3． Conclusion SPINK1 and Spink3 are significantly higher expressed in hepatic tumor tissues and could be used as potential biomarkers for clinical diagnosis． The EＲK pathway may play important roles in up-regulating the expression of Spink3．

Key words: <p>SPINK1, Spink3, EＲK, mTOＲ, Hepatocellular carcinoma</p>