关键词: <, p>, 大鼠, 剖宫取胎, 人工哺乳, 隔离环境, 无菌检查<, /p>

Abstract: Abstract: The clear grade Sprague Dawley rat was purified by cesarean birth and a gnotobiotic population was established by breeding，it will meet requirement of scientific research units，the 35 offspring were obtained by cesarean birth in isolation environment． Two hours later，survival 20 offspring were feed the modified milk by intragastrical gavages and self-designed equipment． The feeding rate was 5 ～ 6 times a day． the 13 offspring were successful wean． These gnotobiotic animals were verified by detecting excrement padding and cotton swab，the gnotobiotic rat model was successful established by germ-free technology and modified milk，which could be conserved by breeding． It solves the problem of shortage of gnotobiotic rat．

Key words: <p>rat, caesarian section, handing feeding, isolator, sterility testing</p>