关键词: <, p>, Mkate2 转染过的大肠杆菌, 肾盂肾炎模型, 红色荧光<, /p>

Abstract: Abstract: Objective An inoculum of 1 × 107 cfu /mL non-pathogenic Escherichia coli-Mkate2 was injected directly into the bilateral renal cortex of the mouse to build the acute pyelonephritis model in normal environment．Method Check the result of model quality with imaging instrument，the method had improved the success of acute pyelonephritis model，and reduce the number of animals used in laboratory tests． Ｒesult It had built the acute pyelonephritis model，the E． coli could not survive in the kidney to reduce the biological risk for the people and environment．

Key words: <p>Escherichia coli-Mkate2, pyelonephritis model, Ｒed fluorescence</p>