关键词: <, p>, 常规实验操作, 福利损伤, NF-&kappa, B<, /p>

Abstract: Abstract: Objective To study the expression of NF-κB in rats’liver after welfare impaired by experimental procedures． Method Male SD rats，welfare were impaired by experimental procedures． Body weight gain and food consumption were recorded throughout the study ，mＲNA of NF-κB p65 in liver were detected by real-time quantitative PCＲ ，and protein expression were detected by Western blotting． Ｒesult Compared with the control group ，the body weight gain and food efficiency ratio ( FEＲ) of experimental group were decreased ( P ＜ 0. 01) ，but mＲNA and protein expression of NF-κB p65 were much higher ，and showed very significant difference ( P ＜ 0. 01) ． Conclusion Welfare impaired caused by the stress of experimental procedures ，the expression of NF-κB in rats’liver are much higher than before． It shows that NF-κB participate in the process of welfare impaired．

Key words: <p>experimental procedures, welfare impaired, NF-κB</p>