关键词: <, p>, ＲAG2, IL2ＲG, 免疫缺陷小鼠, 免疫细胞, 肿瘤移植<, /p>

Abstract: Abstract: Objective To establish aＲAG2 /IL2ＲG double deficient mice model and the preliminary application．Method ＲAG2 deficient mice were crossed with IL2ＲG deficient mice． The genotype of hybrid mice was determined by PCＲ． The percentages of CD3 + ，CD19 + andCD49b + were detected y FCM in terms of its T，B lymphocyte function and NK cell percentage． The physiological and biochemical parameters of blood and major organs weight were observed． Ｒesult ＲAG2 and IL2ＲG deficient mouse were bred respectively，and ＲAG2 / IL2ＲG hybrid mice model was successfully established． Established stable PCＲ identification methods of ＲAG2 / IL2ＲG double genedeficient mice by PCＲ methods． The ＲAG2 /IL2ＲG hybrid mice lacks T cells，B cells，the percentage of NK cells was very low，and the difference was significant ( P ＜ 0. 05) ． Blood biochemical tests showed that compared with the same week-old C57BL /6， serum biochemical parameters of ＲAG2 /IL2ＲG double deficient mice was not significant changes，the difference was not significant ( P ＜ 0. 05 ) ． In the 18 blood physiological indices of ＲAG2 /IL2ＲG double deficient mice，Ｒed blood cell ( ＲBC) and hemoglobin ( HGB) content is low，and the difference was significant ( P ＜ 0. 05) ，White blood cell ( WBC) ，lymphocytes ( LYMPH) ， lymphocyte ratio ( LYM) ，monocytes，neutrophils count ( NEUT) index were significantly lower，and the difference was significant( P ＜ 0. 01) ． The main organ weight displayed the weights of spleen，thymus and mesenteric lymph node are lower than wild-type C57BL /6 mice，the difference was significant ( P ＜ 0. 05) ． The experiments of humanhepatoma cells ( tissue ) transplants show，ＲAG2 /IL2ＲG double gene-deficient mice tumor transplant success rate and growth rate were significantly higher，compared with the parental single gene-deficient mice and the difference was significant( P ＜ 0. 01) ． Conclusion The mice model of ＲAG2 /IL2ＲG double deficient and a stable system of identification are successfully established． The double deficient mouse provide a good animal model for patient-derived tumor xenograft model．

Key words: <p>ＲAG2, IL2ＲG, immunodeficient mouse, immune cells, tumorxenograft</p>