关键词: <, p>, 糖尿病, 脓毒症, 大鼠, TNF-&alpha, 糖原染色<, /p>

Abstract: Abstract: Objective To observe the effect of diabetes mellitus ( DM) on the septic inflammation． Method Diabetic rat model was induced by high glucose and high fat diet and streptozotocin( STZ) injection． On this basis，the sepsis model by the cecal ligation and puncture ( CLP) ． Then the heart tissue and whole blood specimens were harvested and inflammatory factor TNF-α was assayed by ELISA，neutrophil percentage was calculated，and observe the heart tissue was stained by periodic acid-Schiff stain ( PAS) ． Ｒesult ( 1) Compared with normal rats，diabetic rats typically emerged polyuria，polydipsia，polyphagia，and weight loss，with a significant rise in blood glucose and weight loss ( P ＜ 0. 001) ． PAS stain showed myocardial cells staining in normal-control rats，without interstitial cells and blood vessels staining，but cardiomyocytes and interstitial microvessels in diabetic rats all stained． ( 2) In sepsis model based on the diabetes，after CLP the rats showed abnormal activities and mental state; in normal-CLP rats serum TNF-α values were significantly higher than those in normal-control rats ( P ＜ 0. 01) and apparently higher than normal-sham rats ( P ＜ 0. 05) ． In DM-control rats serum TNF-α values were significantly higher than those in normal-control rats ( P ＜ 0. 01) and apparently higher than those in DM-CLP rats ( P ＜ 0. 05) ; In normal-CLP rats peripheral blood neutrophil percentage was significantly higher than those in normal-control and normal-sham rats ( P ＜ 0. 01) ，and in DM-CLP rats peripheral blood neutrophil percentage significantly higher than those in DM-Control and DM-Sham( P ＜ 0. 01) ． Conclusion Inflammatory response of normal rats and DM rats to sepsis is different． This may be related to the difference of immune status in normal and DM rats．

Key words: <p>diabetes mellitus, sepsis, rat, TNF-α, periodic acid-schiff stain</p>