关键词: <, p>, 小肠结肠炎耶尔森氏菌, 假结核耶尔森氏菌, 志贺菌, 空肠弯曲菌, 多重PCＲ<, /p>

Abstract: Abstract: Objective To establish a simple，sensitive，rapid，and the specificity multiplex PCＲ method for Yersinia bacteria， Yersinia pseudotuberculosis， Shigella and Campylobacter jejuni from laboratory animals．Method According to the fox gene of enterocolitis Yersinia and inv of Yersinia pseudotuberculosis，ipaH gene of Shigella and gyrA gene of Campylobacter jejuni，we designed and screened a pair of specific primers． The annealing temperature and other conditions were optimized，then the specificity and sensitivity of The Multiplex PCＲ were analysis． At last，the multiplex PCＲ method was use to detect the samples from mouse and guinea pig． Ｒesult The multiplex PCＲ amplified the expected PCＲ product， namely Yersinia enterocolitis ( 511bp ) ， Yersinia pseudotuberculosis ( 779 bp) ，Shigella ( 290 bp) and Campylobacter jejuni ( 156 bp) ． The sensitivity of this method is 1 × 10 － 3 ng /μL ( Yersinia bacteria，Yersinia pseudotuberculosis，Shigella) and 1 × 10 － 2 ng /μL ( C． jejuni) ． No specific positive was detected from the other samples． Conclusion The multiplex PCＲ method has good application and development prospects in detection of microorganisms in laboratory animals．

Key words: <p>Yersinia enterocolitica, Yersinia pseudotuberculosis, Shigella, Campylobacter jejuni, multiplex PCＲ</p>