关键词: <, p>, 经皮电阻试验, 皮肤腐蚀性, 替代<, /p>

Abstract: Abstract: Objective To assess modified in vitro transcutaneous electrical resistantce test method． Method 28 ～30 day-old rats are humanely killed，the dorso-lateral skin of each animal is then removed． Skin discs with a diameter of approximately 20 mm each，are removed． If the resistance value is greater than 20 kΩ，the discs could be used for the test method． Test chemicals ( 150 μL) are applied uniformly to the epidermal surface． Three skin discs are used for each test． Test chemicals are applied for 24 hours at room temperature，and then are removed by washing． The skin impedance is measured as TER． If TER values produced by such chemicals are less than or around 15 kΩ，an assessment of dye penetration should be carried out． The optical density ( OD) of the incubation solution is measured at 565nm． The sulforhodamine B dye content per disc is calculated from the OD values． Result The sensitivity，specificity and accuracy of the modified TER test method are 100%，83% and 88% respectively． The TER test method is considered valid to discriminate corrosive from non-corrosive chemicals．Conclusion The modified TER test method is valid in our Lab．

Key words: <p>TER, skin corrosion, replacement</p>