关键词: 高脂饲料, 饲喂方式, SD 大鼠, Lee's 指数

Abstract: Abstract: Objective To establish high fat diet induced obesity in male SD rat model of nutrition． Method Four weeks old male SD rats were randomly divided into experimental group( n = 24) and control group( n = 6) ． The control group was fed normal diet and the experimental group was fed high fat diet which was composed with normal diet ，egg yolk and peanuts． Two weeks later，the obesity resistant rats were excluded from the experimental group．Weight and Lee's index were observed after eight weeks． After twelve hours fasted ，all rats were sacrificed and tested for TC、TG． The hepatic tissue of rats was analyzed histomorphologically． Ｒesult Experimental group weighted 526. 6 ± 46. 4g and the control group weighted 415. 6 ± 33. 7g， there for the weight of experimental group rats were 33. 1% higher than control group rats． Lee's index of the experimental group was 330. 0 ± 8. 2，while the control group was 307. 0 ± 6. 6． The serum total cholesterol of experimental group was 2. 16 ± 0. 17 mmol /L，serum triglyceride was 1. 40 ± 0. 34 mmol /L and control group，s serum total cholesterol was 1. 66 ± 0. 17 mmol /L，serum triglyceride was 0. 82 ± 0. 13 mmol /L． Pathology results showed the experimental group were induced fatty liver．Conclusion This study showed that feeding high fat diet which was composed with normal diet ，egg yolk and peanuts could successfully and stably established obesity model．

Key words: high fat diet, feeding mode, SD rats, Lee's index