Aβ1－40脑内注射对大鼠海马Na +-K + -ATP 和Ca2 +- ATP 酶活性的影响及加减地黄饮子的干预作用

实验动物科学 ›› 2015, Vol. 32 ›› Issue (03): 27-29.

Aβ1－40脑内注射对大鼠海马Na +-K + -ATP 和Ca2 +- ATP 酶活性的影响及加减地黄饮子的干预作用

( 1．黑龙江省齐齐哈尔医学院医药科学研究院，齐齐哈尔161006)( 2．黑龙江中医药大学基础医学院，哈尔滨市150040)

出版日期:2015-06-29 发布日期:2017-04-13

The Effect of Modified Decoction of Ｒehmanniae on Na +-K+-ATP and Ca2 +-ATP Enzyme Activity in Ｒat Models of Alzheimer’s Disease

Online:2015-06-29 Published:2017-04-13

摘要/Abstract

摘要： 摘要: 目的观察加减地黄饮子对Aβ1 － 40脑内注射对大鼠脑组织Na +-K +-ATP 酶的影响。方法以大鼠海马注射β淀粉样蛋白导致的老年性痴呆模型，并以正常老龄鼠为空白对照，西药脑复康为阳性对照，对加减地黄饮子预防组和加减地黄饮子治疗组从Na + -K + -ATP 和Ca2 + -ATP 酶活性的影响进行了研究。结果加减地黄饮子具有提高三磷酸腺苷酶活性的作用。结论提高三磷酸腺苷酶活性的可能是加减地黄饮子防治老年性痴呆的机制之一，为补肾活血化痰开窍治疗老年性痴呆的观点提供了部分实验依据。

关键词: 加减地黄饮子, Na +-K + -ATP, Ca2 +-ATP 酶, 酶活性

Abstract: Abstract: Objective To investigate the effect of Modified Decoction of Ｒehmanniae on the Na +-K +-ATP and the Ca2 +-ATP enzyme activities in rat models of Alzheimer's disease ( AD) ． Method Ｒat AD model was induced by sterotaxis injection of amyloid － β1 － 40 in bilateral hippocampus． The aging rats were used as the blank control and the models treated with piracetam were used as the positive control． The Na +-K +-ATP and the Ca2 + -ATP enzyme activities in brain were assayed by biochemical techniques．Ｒesult The Modified Decoction of Ｒehmanniae improved the activities of the Na +-K +-ATP esterase and the Ca2 + -ATP esterase． Conclusion The results suggest that the Modified Decoction of Ｒehmanniae improves the activities of the Na +-K +-ATP esterase and the Ca2 + -ATP esterase，and can be used as an effective drug for Alzheimer's disease．

Key words: Modified Decoction of Ｒehmanniae, Na +-K +-ATP, Ca2 +-ATP enzyme, enzyme activity

中图分类号:

R285.5

牛英才, 谢宁 . Aβ1－40脑内注射对大鼠海马Na +-K + -ATP 和Ca2 +- ATP 酶活性的影响及加减地黄饮子的干预作用[J]. 实验动物科学, 2015, 32(03): 27-29.

[1]	祝明皓,陆涛峰, 陈洪岩 . DNA 稳定的银纳米簇用于检测端粒酶活性研究[J]. 实验动物科学, 2015, 32(03): 16-18.
[2]	杨善民,陈福,陈美玉. 移植瘤株冷冻保护的实验研究[J]. 实验动物科学, 1997, 14(01): 21-25.