关键词: 新型呼肠病毒, 抗原蛋白, 受体蛋白

Abstract: Abstract:Objective To study the key fragment which was importent for the interaction between S1 gene of a new type reovirus and its cell receptor, and provide the basis for receptor screening. Method Three fragments,which coding full-length S1 and N and C terminal of S1 gene were cloned to pIRSE2-EGFP vector to get recombinant plasmids pGS, pGSN and pGSC. L929 and Hela cells were transfected with three recombinant plasmids, respectively, and the fragment which was involved for interaction of S1 gene and receptor was indicated by analysis the expression of reporter gene EGFP. Result When transfecting with equal amount of three recombinant plasmids, pGSN-treatment group showed the highest level of EGFP expression and pGSC-treatment group produced the lowest EGFP expression. Moreover, when co-transfecting with pGS and pGSN at different ratio, the higher ratio of pGSN the admixture comtained, the higher level of EGFP expression could generate. Conclusion N terminal of S1 gene (62-406 bp) was essential for interaction between S1 gene and its cell receptor.

Key words: new type of reovirus, antigen protein, receptor protein