15-KETE 对大鼠肺动脉平滑肌细胞Kv1. 5、Kv2. 1 表达的影响

实验动物科学 ›› 2013, Vol. 30 ›› Issue (05): 9-14.

15-KETE 对大鼠肺动脉平滑肌细胞Kv1. 5、Kv2. 1 表达的影响

( 1．哈尔滨医科大学第二临床医学院实验动物中心，哈尔滨150086) ( 2．哈尔滨医科大学公共卫生学院职业卫生与职业医学教研室，哈尔滨150086) ( 3．哈尔滨医科大学第二临床医学院药学部，哈尔滨150086) ( 4．哈尔滨医科大学院药学院，哈尔滨150086)

出版日期:2013-10-31 发布日期:2014-05-13
基金资助:
黑龙江省科技攻关项目( No． PC09S03)

The Effect of 15-KETE on the Protein and mＲNA Expression of Kv1. 5，Kv2. 1 in Ｒat Pulmonary Arterial Smooth Cells

Online:2013-10-31 Published:2014-05-13

摘要/Abstract

摘要： 摘要: 目的从细胞分子水平研究电压门控型钾通道亚型Kv1. 5、Kv2. 1 在15-酮基二十碳四烯酸( 15- ketoeicosatetretraenoic acid， 15-KETE) 致大鼠肺动脉收缩过程中的作用。方法通过酶法分离、培养SD 大鼠肺动脉平滑肌细胞( pulmonary arterial smooth cells，PASMCs) ，使用ＲT-PCＲ和Western blot 技术观察15-KETE 对大鼠 PASMCs 上Kv1. 5、Kv2. 1 通道表达的影响。结果( 1) 15-KETE 下调大鼠PASMCs 膜上Kv1. 5 通道mＲNA 及蛋白质的表达; ( 2) 15-KETE 下调大鼠PASMCs 膜上Kv2. 1 通道mＲNA 及蛋白质的表达。结论Kv1. 5、Kv2. 1 通道参与由15-KETE 引起的缺氧肺动脉收缩( hypoxic pulmonary vasoconstriction，HPV) 。

关键词: 15-酮基二十碳四烯酸, 缺氧肺动脉收缩, 电压门控性K + 通道亚型, 肺动脉平滑肌细胞

Abstract: Abstract: Objective To observe the effect of Kv1. 5、Kv2. 1 channels in rat pulmonary arterial smooth cells on the process of pulmonary vasoconstriction induced by 15-KETE． Method Western blot and PCＲ were used to identify the effect of 15-KETE on expression of Kv1. 5、Kv2. 1 channels in cultured rat pulmonary arterial smooth muscle cells ( PASMCs) ．Ｒesult ( 1) The expressions of Kv1. 5 mＲNA and protein in rat PASMCs were significantly down-regulated by 15-KETE． ( 2) Theexpressions of Kv2. 1 mＲNA and protein in rat PASMCs were significantly down-regulated by 15- KETE． Conclusion Kv1. 5、Kv2. 1 channels are involved in vasoconstriction induced by 15-KETE in rat PA．

Key words: 15-ketoeicosatetretraenoic acid, hypoxic pulmonary vasoconstriction, voltage-gated potassium channel
subtype, pulmonary arterial smooth cells

中图分类号:

Ｒ963

郭守利, 孙艳, 张一飞, 朱大岭. 15-KETE 对大鼠肺动脉平滑肌细胞Kv1. 5、Kv2. 1 表达的影响[J]. 实验动物科学, 2013, 30(05): 9-14.

GUO Shou-Li, SUN Yan, ZHANG Yi-Fei, ZHU Da-Ling. The Effect of 15-KETE on the Protein and mＲNA Expression of Kv1. 5，Kv2. 1 in Ｒat Pulmonary Arterial Smooth Cells[J]. laboratory animal science, 2013, 30(05): 9-14.

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[2]	郭守利, 邹悦, 张一飞, 刘晔, 朱大岭. 15 － KETE 对大鼠肺动脉平滑肌细胞Kv1. 2、Kv3. 4 表达的影响[J]. 实验动物科学, 2013, 30(03): 19-24.