关键词: 心肌缺血再灌注损伤, 芬太尼联合东莨菪碱, 大鼠

Abstract: Abstract: To study the protective effects of pretreatment with fentanyl combine scopolamine on myocardial ischemia and reperfusion injury in rats． Twenty-four SD rats were randomly divided into three groups( n = 8 in each group) : control group ( group c，acute myocardial ischemia and reperfusion injury group) 、group F ( fentanyl group，the rats intravenously injected with fentanyl 0. 05mg /kg at 10min before myocardial ischemia) and group F + D ( fentanyl + scopolamine group，the rats intravenously injected with fentanyl 0. 05mg /kg and scopolamine 0. 3mg /kg at 10min before myocardial ischemia) ． Heart rate ( HＲ) ，left ventricular systolic pressure ( LVSP) ，left ventricular end diastolic pressure ( LVEDP) ，maximum positive and minimum negative left ventricular pressure derivatives ( ± dp / dtmax ) were continuously monitored and recorded at baseline，30min after ischemia，30min and 120min after reperfusion process． Contents of superoxide dismutase ( SOD) ，malonyldialdehyde ( MDA) and NO level in artery blood of rats were detected after reperfusion 120min． There were no significant difference in HＲ and LVEDP at any measurement point during the experiment among all groups ( P ＞ 0. 05) ． LVSP and ± dp /dtmax were significantly higher in group F than in group C at 30min、120min after reperfusion ( P ＜ 0. 05 ) ，and these were as well significantly higher in group F + D than in group F( P ＜ 0. 05) ． The serum activity of SOD was no significant difference in all groups ( P ＞ 0. 05) ． And the serum concentration of MDA was significantly lower in group F + D than in group F and group C ( P ＜ 0. 05) ． The serum concentration of NO was significantly higher in group F and group F + D than in group C ( P ＜ 0. 05) ． The arrhythmia score was significantly lower in group F + D than in else groups( P ＜ 0. 05) ． The protective effects of pretreatment with fentanyl combine scopolamine on myocardial ischemia and reperfusion injury in rats was better than fentanyl single．

Key words: myocardial ischemia and reperfusion injury, fentanyl combine scopolamine, rat