关键词: 同种反应性, 肝移植, 免疫耐受

Abstract: Abstract: Objective To investigate whether infusion of alloreactive lymphocytes induced in vitro can prolong the survival of liver allograft in rats，so as to find out a new method to induce liver transplantation tolerance． Method Lymphocytes from Lewis rat were used as stimulating cells，lymphocytes from BN rat were stimulated by these stimulating cells and expanded in vitro; the proliferation of BN lymphocytes were determined by CFSE method． The proliferated BN lymphocytes were used as idiotype vaccine to vaccinate BN rats，then liver allograft from Lewis rats were transplanted to BN rats． Ｒesult Alloreactive lymphocytes proliferated vigorously in vitro; infusion of alloreactive lymphocytes induced in vitro can prolong the survival of liver allograft significantly in rats． Conclusion infusion of alloreactive lymphocytes induced in vitro may be a new method to induce donor specific transplantation tolerance．

Key words:  alloreactivity, liver transplantation, immune tolerance