关键词: 颅脑损伤, 电子颅脑损伤仪, 大鼠模型

Abstract: Abstract: Objective To study the TBI rats model produced by eCCI and provide a model establishing method of easy operation，the controllable degree of damage，good repeatability for the TBI experimental study． Method 65 SD rats，which were randomly divided into blank control group A，model group B，model group C，model group D and sham-operation group E，were produced as the animal model of brain injury in different degree by eCCI according to different selected parameters． The speed was 3 m/s and the depth was 3 mm in model group B． The speed was 4 m/s and the depth was 3 mm in model group C． The speed was 5 m/s and the depth was 3 mm in model group D． The quality of the model was evaluated through detecting the dynamic changes of S100B and NSE by ELIASA in animal serum． Ｒesult The content of S100B and NSE in serum was increased on the first day， second day，third day after animal damaged in model group by ELISA． Between the model group and sham operation group，there were significantly different ( P ＜ 0. 01) ． In model group B，C and D，the content of S100B and NSE was increased significantly with the increase of hit ( P ＜ 0. 01) ． Conclusion By using eCCI TBI rat model can be established with regulation of hit speed and depth． The damage of model was related to the hit speed． The method of TBI model preparation has the advantages of easy operation and control，better repeatability，and can be used according to the need to make various animal model for the study．

Key words: brain injury, eCCI, brain injury, eCCI;