关键词: 健脾化湿颗粒, 肠易激综合征, 内脏敏感性, CＲF, CＲF-Ｒ1

Abstract: Abstract: Objective To explore the effects of Jianpi Huashi Granule on visceral sensitivity and CＲF /CＲF-Ｒ1 expression in brain of D-IBS model rat． Method Seventy-two male Sprague-Dawley rats were equally and randomly divided into six groups: normal group，model group，dicetel group，low-，medium-，and high-dose Jianpi Huashi Granule groups; D-IBS was induced by restraint stress combined with administration of sennae decoction; The visceral sensitivity of rats was evaluated by colorectal distention，and recorded the water injection rate when the score of abdominal withdrawal reflex( AWＲ) was 3． The expression of CＲF and CＲF-Ｒ1mＲNA in the brain was detected by PCＲ． Ｒesult Compared with normal group，the water injection rate of the model group showed a significant decrease when the score of AWＲ was 3( P ＜ 0. 01) ，and the expression of CＲF and CＲF-Ｒ1mＲNA in the brain significantly increased ( P ＜ 0. 01) ; compared with model group， the water injection rate of the dicetel group，medium-，and high-dose Jianpi Huashi Granule groups showed a significant increase when the score of AWＲ was 3( P ＜ 0. 01) ，and the expression of CＲF and CＲF-Ｒ1mＲNA in the brain significantly decreased( P ＜ 0. 01) ． Conclusion medium-，and high-dose Jianpi Huashi Granule groups reduces visceral sensitivity in the rats with DIBS possibly by modulating the expression of CＲF and CＲF-Ｒ1mＲNA in the brain．

Key words: Jianpi Huashi Granule, irritable bowel syndrome, visceral sensitivity, CＲF, CＲF-Ｒ