关键词:  , 酸水解法, 索氏抽提法, 膨化饲料, 粗脂肪

Abstract: Abstract: Objective To investigate proper determination method for crude fat in extruded feed． Method Crude fat contents in experiment feeds including non-extruded dog feed，extruded dog feed，non-extruded primate feed， extruded primate feed，non-pelleted rodent feed，pelleted rodent feed，non-pelleted rabbit feed and pelleted rabbit feed were determined by acid hydrolysis method and Soxhlet extraction method， respectively． Result The determined level of crude fat in extruded primate feed by acid hydrolysis method was very significantly higher than that by Soxhlet extraction method ( P ＜ 0. 001) ． The determined level of crude fat in extruded dog feed by acid hydrolysis method was significantly higher than that by Soxhlet extraction method ( P ＜ 0. 05 ) ． However，the determined levels of crude fat in non-extruded primate feed and non-extruded dog feed had no differences between two the methods( P ＞ 0. 05) ，and The determined levels of crude fat in pelleted feeds and non-pelleted feeds also had no differences between the two methods( P ＞ 0. 05) ． The loss of crude fat determined by acid hydrolysis method was less than that by Soxhlet extraction method during feed processing． Conclusion The acid hydrolysis method is suitable for determining crude fat in extruded feed．

Key words:  acid hydrolysis, Soxhlet extraction, extruded feed, crude fat