关键词: 胰岛素样生长因子-1, 精子介导转基因, 转基因小鼠

Abstract: Abstract: Objective To establish transgenic mice brain-specifically expressing insulin-like growth factor -1 ( Insulin-Like Growth Factors，IGF-1 ) ． Method Sperm was used for gene transduction as a carrier，postnatal mice were detected by PCR assay to establish transgenic mouse lines． Western blot and immunofluorescence were applied to detect the IGF-1 expression level in transgenic mouse subgranular zone( SGZ) and the amount of reporter gene EGFP positive cells． Result Three positive mice were obtained，2 of them could be inherited stably，and established transgenic mouse lines． The IGF-1expression level in transgenic mouse SGZ was higher than that in the normal mice，meanwhile，EGFP could be expressed in the same area． Conclusion IGF-1 transgenic mice were prepared successfully．

Key words: IGF-1, sperm-mediated gene transfer, transgenic mouse