关键词: 奶牛乳腺炎, 金黄色葡萄球菌, 小鼠乳腺炎模型

Abstract: Objective Infect mouse mammary gland with Staphylococcus aureus(S．aureus)isolated and purified from clinical mastiffs milk samples in order to establish a mouse model of Staphylococcus aureus of dairy COWS mastitis．Methods 8～10 days postpartum far inbred ICR female mice were randomly divided into three groups， including the sterile saline control group，tow—dose—bacteria—attack group(2×1 0² CFU of Staphylococcus aureus) and high-dose—bacterial—attack group(5×10⁶ CFU of Staphylococcus aureus)．Staphylococcus aureus or sterile saline were injected into the fourth pair mammary gland through the nipple tube with non—invasive method．24 h after bacteria attack，milk of these rats was collected to do white blood cell count，and blood from eyes to do blood testing and flow cytometry；after the female mice were killed，taking the breast tissue to do homogenate bacterial count，and do biopsy．Results The high—dose group have a significant or very significant difference(P<0．05) with the control group whether in the histological observation and body temperature，or milk white blood cell count，breast homogenate count，some indicators in blood testing and flow cytometry．But compared the low—dose group with the control group，indicators have no significant differences except the blood CD4+／CD8+ratio was significantly different(P<0．05)．Conclusion The distant strains of mice models of S．aureus mastitis was successfully established when the concentration of Staphylococcus aureus is 5×1 00 CFU．bacterial attack for 24 h。 and concentration of 2×10² CFU of bacteria is not suitable for the establishment of distant strains of mice models of S．aureus mastitis．

Key words: cow mastiffs, staphylococcus aureus, mice models of mastitis