关键词: 成骨细胞, 多聚赖氨酸(PLL, 鼠尾胶原, 贴壁

Abstract: Objective To compare polylysine and d collogen made by rat’s tail affecting on sticking to the wall of culture dishes in vitro. Methods We paved culture dish’s wall with polylysine and collagen made by rat’s tail, then to planted osteoblast. At 24h 48h 72h, we observed, under microscope, the cell’s morphous and area percentage which they stuck to the wall of culture dishes. Result In the polylysine group, at 24h, 48h, 72h, osteoblast adherence displayed hypodispersion and adherence area ratio is 30.6%±12.9%, 43.8%±14.5%, 56.3%±15.4%, respectively. In the collagen made by rat’s tail group, at 24h 48h 72h, the cell adherence area ratio is 42.5%±15.7%, 61.3%±17.1%, 94.4%±14.1%, respectively. At 24h, the cell adherence displayed mass; at 48h, the cell displayed mass and conjunction each other; at 72h, the cell displayed plaque. Two groups have significant difference. Conclusion The results showed that collagen made by rat’s tail is much suitable for osteoblast sticking wall growth.

Key words:  Osteablast culture, Polylysine, Rat tail’s collagen, Sticking wall comparison