关键词: 丙型肝炎, 免疫应答, 核酸疫苗, 多肽疫苗, 树突状细胞疫苗

Abstract: Hepatitis C is an important infectious disease threatening human health．Because of the most heterogeneous and high mutational rate of the HCV genome，the virus can easily escape from the immune recognition of the host，thus it is difficult to develop a vaccine，as theory and technique of molecular biology rapidly develop， gene immune technique now is applied into HCV vaccine development．Recombinant plasmid pEF-HCV—C constructed by inserting a highly conserved HCV—core gene fragment with the highly expressed eukaryotic expression vector pEF．By direct intramuscular immunization，DNA-based immunization has successfully induced specific humeral immune response against HCV-core protein．Nucleic acid vaccine have a number of advantages over conventional vaccines including the ability to induce a wider range of immune response types，this vaccine expressing HCV—core is a potential candidate for a prophylactic vaccine for humans．Dendritic cells are capable of eliciting a vigorous antiviral response in native T cells．The administration of DC offers a potential approach to induce high—level immunity against hepatitis C virus．Vaccination with protein-transduced DC may constitute an important antiviral strategy for hepatitis C virus．

Key words: Hepatitis C, Immune response, Nucleic acid vaccine, Peptide vaccine, Dentritic cell vaccine